• Single Nucleotide Polymorphisms
• Length Polyorphisms
• Null Mutations

  The WSU Genomics Facility Genotyping Laboratory is a state of the art facility capable of high throughput genotyping using several advanced technologies. The Genotyping Laboratory recently purchased an ABI 7900HT and an ABI 3100 increasing the throughput for several genotyping applications. The ABI 3100 capillary electrophoresis system allows the facility to genotype fragment length polymorphisms (GeneScan), single nucleotide polymorphisms-SNPs-(SNaPshot), and small scale sequencing projects. The 16 capillary system can genotype 288 samples for 8+ multiplexed fragment length polymorphisms allowing the generation of over 2300 genotypes a day. When used with the SNaPshot multiplexed SNP detection system, the 3100 can genotype 384 samples for 12+ SNPs allowing the generation of over 4600 genotypes a day. In addition, the 3100 allows ultra rapid sequencing of small to medium DNA fragments in small batches. The ABI 7900HT Sequence Detection System is the flagship instrument for quantitative and real time PCR. With the installed stacker robot, the 7900HT is capable of performing over 5000 real time and/or quantitative PCR reactions a day. Combined ABI Prism Taqman primers and probes, the system is at the leading edge of Q-PCR and RT-PCR. The 7900HT is also the ideal system for validating gene expression results from microarray experiments. Using ABI Prism Taqman primers and probes also allows us to perform approximately 1200 SNP genotypes a day using the 7900HT's allelic discrimination functionality. Finally, Applied Biosystems Assays on Demand and Assays by Design services allow us to offer both custom and commercially available assays for gene expression and SNP detection that are reliable, readily available and affordable.

AGTC Project Sample Submission Form (mandatory) (PDF)